Fig. 1.

Isolation and culture of suture mesenchymal stem cells. (A) Craniofacial computed tomographic scan with three-dimensional reconstruction of three patients before operation showed typically right coronal suture fusion. Representative H&E staining of contralateral patent suture (B) and prematurely fused suture (C). (D) The tissues, containing fused or contralateral cranial suture, in the culture dishes. (E) The cells were migrating from the cranial suture tissue. (F, G) Inverted microscopic view of suture mesenchymal cells exhibiting almost homogeneous spindle morphology and stained with 0.25% crystal violet blue.