Fig. 3.

Expression of osteogenic phenotypic genes and FGFR family genes in suture cells. (A-C) Osteogenic differentiation in the mesenchymal stem cells, from normal patent sutures (Normal group) or prematurely synostosed sutures (Synostosed group), was tested. Con, control group without osteogenic induction; Ost, osteogenic differentiation group with osteogenic induction. (A) Representative pictures of Alizarin Red S staining. Both Cells were cultured with osteogenic induction media for six days. (B, C) mRNA levels of Runx2 and OCN by RT-PCR analysis. (D∼G) RT-PCR analysis of BMP3, FGF2, FGFR2 and FGFR3 in normal and synostosed sutures-derived mesenchymal cells. Results are mean±SD. *p<0.05, **p<0.01, and ***p<0.001 versus normal cells. OCN, osteocalcin.