Table 1.
Sequences of RT-PCR primers
| Gene name | Sequences | Size (bp) | |
|---|---|---|---|
| Runx2 | Forward: | 5'-CACCTTGACCATAACCGTCTTC-3' | 196 |
| Reverse: | 5'-CGGGACACCTACTCTCATACTG-3' | ||
| OCN | Forward: | 5'-GACTGTGACGAGTTGGCTGA-3' | 233 |
| Reverse: | 5'-GCCCACAGATTCCTCTTCTG-3' | ||
| BMP3 | Forward: | 5'-TCAGGCCGTAGATCATTGTTG-3' | 215 |
| Reverse: | 5'-AGGTAACACAGTGCCTTTCACA-3' | ||
| FGF2 | Forward: | 5'-AAGACCAACCTGGTGAAACC-3' | 151 |
| Reverse: | 5'-TTATGGCTCACTGCAACCTT-3' | ||
| FGFR2 | Forward: | 5'-GGATAACAACACGCCTCTCTTC-3' | 155 |
| Reverse: | 5'-CATGACCACTTGCCCAAAG-3' | ||
| FGFR3 | Forward: | 5'-GGAGGTGATTCCAGTGAAGATA-3' | 150 |
| Reverse: | 5'-TGGGACACACAGCAATTAGAAG-3' | ||
| Bmi1 | Forward: | 5'-GCTGCTCTTTCCGGGATT-3' | 184 |
| Reverse: | 5'-TGCTGGTCTCCAGGTAACG-3' | ||
| Lef1 | Forward: | 5'-CCTTGGTGAACGAGTCTGAA-3' | 237 |
| Reverse: | 5'-TGGGTGGAGAAAGAGATCCA-3' | ||
| Gli1 | Forward: | 5'-AGGAGTTCGTGTGCCACTG-3' | 239 |
| Reverse: | 5'-GGCATTGCTGAAGGCTTTAC-3' | ||
| Nanog | Forward: | 5'-CACTACGACCCAGGCTTCAT-3' | 234 |
| Reverse: | 5'-CCACAGCCACATCTGTAACTTC-3' | ||
| Axin2 | Forward: | 5'-TTGAGCTAGGAGTGCGTTCA-3' | 206 |
| Reverse: | 5'-CAACATGGTCAACCCTCAAG-3' | ||
| GAPDH | Forward: | 5'-CAACATGGTCAACCCTCAAG-3' | 197 |
| Reverse: | 5'-CAACATGGTCAACCCTCAAG-3' |