Fig. 6.

Identification of linear epitopes in the RBD region of SARS-CoV-2. a Detection of peptide binding to the antibodies in sera from mice immunized with 10 µg at 35 days post 1st immunization using a chip coated with overlapping peptides that covered the RBD of SARS-CoV-2. b–e ELISA assay to detect the binding capacity of mouse antisera to four positive peptides in the chip. f The neutralization blocking effects of the four peptides and RBD-Fc protein. Total IgG from the pooled sera immunized with RBD-Fc was mixed with the four peptides, respectively, and then the remaining neutralization effects of the IgG were detected using SARS-CoV PsV assay. g The inhibitory activities of the four peptides and the RBD-Fc protein against SARS-CoV-2 PsV