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. 2020 Nov 26;10:20598. doi: 10.1038/s41598-020-75833-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SMYD3 cellular distribution correlates with the lysine trimethylation (Kme3). (a) The increased nuclear distribution of SMYD3-HA-FLAG on square patterns (green dots; n = 105) correlates with higher nuclear staining for lysine tri-methylation marks (Kme3). Conversely, rectangle patterns (blue and grey spots; n = 68 and 37, respectively) have higher SMYD3 and Kme3 levels in the cytoplasm. (b) Confocal microscopy images of a C2C12 cell spread on a square micropattern, showing the co-localisation of SMYD3-HA-FLAG (green) and lysine tri-methylation Kme3 (red) marks. The magnified square highlights SMYD3/Kme3 colocalisation. Scale bars: 30 µm. (c) Upper panels: Detailed representation of the SMYD3 and Kme3 co-localisation within (i) the cytoplasm and (ii) the nucleus for a cell spread on a square micropattern. Lower panels: the same quantification for a cell spread on a rectangle micropattern showing cytoplasmic (iii) and nuclear (iv) quantification. (d) Graphical representation of the correlation (Pearson coefficient) between SMYD3 and Kme3 lysine tri-methylation localisation. n = numbers of individual cells measured: square n = 105, rectangle 1:5 n = 68, rectangle 1:8 = 37. (e) Graphical representation showing a quantified lack of correlation (Pearson coefficient) between SMYD3 and Kme2 lysine di-methylation localization. n = numbers of individual cells measured: square n = 24, rectangle 1:5 n = 18, rectangle 1:8 n = 19.