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. 2020 Oct 25;10(11):2117. doi: 10.3390/nano10112117

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) AlamarBlue^TM metabolic activity of human osteoblasts (HOBs) cultured on anodised titanium samples with respective pore diameter at Day 7 and 14 in Osteoblast Growth Medium. Values are mean ± S.D., n = 4, * p < 0.05, **** p < 0.001; (b) DNA content of human osteoblasts (HOBs) measured using PicoGreen assay, cultured on anodised titanium samples with respective pore diameter at Day 14 in Osteoblast Growth Medium; (c) Normalised alkaline phosphatase (ALP) activity per DNA of human osteoblasts (HOBs) cultured on anodised titanium samples with respective pore diameter at Day 14 in Osteoblast Growth Medium. For (b,c) Values are mean ± S.D., n = 4, * p < 0.05; LIVE/DEAD cell viability microscopy (d–f) with green stained live cells and immunofluorescence microscopy (g,h) with cell nuclei (blue), F-actin cytoskeleton (green) and vinculin (red) of human osteoblasts (HOBs) cultured on anodised titanium samples with respective pore diameter at Day 14 in Osteoblast Growth Medium. Scale bar = 100 µm.