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. 2020 Oct 31;12(11):3206. doi: 10.3390/cancers12113206

Figure 3.

Figure 3

Impact of laser-microdissection on mRNA expression levels of hENT-1 in PDAC samples (A) Representative pictures of PDAC epithelium and stroma before and after laser-assisted microdissection, performed on frozen tissue with the Leica LDM7000 microscope, as described previously [62]. (B) Comparison between hENT-1 gene expression values in microdissected (LMD) and non-microdissected (no LMD) samples from 22 PDAC tissues. Expression of hENT-1 was evaluated by Real-Time Quantitative PCR and normalized to GAPDH expression, as described previously [42]. t-test statistics was performed using Graph Pad version 7. Clinicopathological features of this subgroup of patients are reported in Table S1 [64,65,66]. (C) Comparison between hENT-1 protein expression values (evaluated by nanoLC-MS/MS) in LMD stromal and tumor compartments (* = p < 0.05) in n = 15 matched samples with respective survival curves. Protein values are represented in log10(LFQ). Patients were grouped in high and low hENT-1 protein expression according to the median cutoff. [62,67]. T-test statistics and Kaplan-Meier plots were performed in R version 3.5.2.