Table 2.
Number of neurons (left) and astrocytes (right) measured, the % of these showing activity, the total number of fluctuations analyzed under each condition. and the mean and standard deviation for the amplitude (mM) and duration (minutes) for the analyzed fluctuations.
| Neurons | Astrocytes | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cells (n) | % Cells (n) Active | Fluctuation (n) | mM Mean | mM SD | p Values | Min Mean | Min SD | Cells (n) | % Active | Fluctuation (n) | mM Mean | mM SD | p Values | Min Mean | Min SD | |
| Control | 412 | 21.6 | 166 | 2.2 | 1.2 | 9.2 | 4.1 | 213 | 43 | 166 | 2.1 | 1.4 | 8.6 | 3.2 | ||
| TTX | 94 | 7.4 | 7 | 0.7 | 0.3 | ** | 8.2 | 3.7 | 39 | 35.9 | 23 | 1 | 0.3 | *** | 8.7 | 4.1 |
| BIC | 76 | 19.7 | 32 | 0.8 | 1 | *** | 6.6 | 2.3 | 22 | 59.1 | 20 | 2.7 | 1.9 | 0.06 | 8.1 | 2.3 |
| CGP | 101 | 12.9 | 20 | 2 | 0.6 | 0.48 | 10.9 | 3.5 | 49 | 44.9 | 42 | 2.1 | 1.2 | 0.78 | 9.6 | 4.6 |
| SNAP/NNC | 53 | 22.6 | 17 | 1.1 | 0.2 | *** | 10.2 | 3.1 | 86 | 34.9 | 33 | 3.2 | 2.0 | ** | 10.8 | 6.2 |
| NPA | 70 | 15.7 | 20 | 1 | 0.3 | *** | 10.7 | 3.8 | 43 | 32.6 | 31 | 1.3 | 0.6 | ** | 9.4 | 3.6 |
| Sarcosine | 74 | 20.3 | 20 | 1.5 | 0.5 | 0.02 | 11.3 | 3.7 | 62 | 21 | 25 | 2.6 | 1.7 | 0.06 | 8.5 | 3.5 |
| TFB-TBOA | 36 | 27.8 | 10 | 1.9 | 1.7 | 0.69 | 8.5 | 3.7 | ||||||||
| CNQX | 130 | 6.2 | 9 | 2.6 | 1.3 | 0.29 | 9.6 | 4.1 | 45 | 37.8 | 25 | 2.1 | 1.1 | 0.86 | 8.9 | 3.1 |
| APV | 156 | 10.3 | 19 | 2.5 | 0.9 | 0.22 | 10.9 | 5.2 | 52 | 28.8 | 19 | 2.7 | 2.5 | 0.34 | 13.1 | 4.9 |
| MPEP | 117 | 16.2 | 30 | 1.9 | 0.7 | 0.28 | 9.5 | 3.8 | 26 | 46.2 | 53 | 3.9 | 2.2 | 0.36 | 9.1 | 6.2 |
| α-BT | 118 | 11.9 | 29 | 1.6 | 0.6 | 0.02 | 10.1 | 3.7 | 43 | 67.4 | 89 | 2 | 1.5 | 0.93 | 7.3 | 3.7 |
| Atropine | 124 | 16.1 | 28 | 2.6 | 1.3 | 0.14 | 8.5 | 2.9 | 73 | 49.3 | 74 | 2.5 | 1.2 | 0.02 | 7.7 | 2.9 |
| PPADs | 70 | 11.4 | 19 | 1.4 | 0.9 | 0.01 | 10.7 | 4.9 | 57 | 24.6 | 24 | 1.6 | 0.6 | 0.1 | 8 | 2.4 |
| Caffeine | 142 | 22.5 | 75 | 1.9 | 0.7 | 0.05 | 9.4 | 4.8 | 32 | 62.5 | 33 | 2 | 0.9 | 0.84 | 8.8 | 3.2 |
| Prazosin | 111 | 13.5 | 21 | 1.3 | 0.4 | ** | 9.7 | 3.7 | 81 | 40.7 | 50 | 1.7 | 0.8 | 0.18 | 10.4 | 4.6 |
| Propranolol | 123 | 7.3 | 11 | 1.9 | 0.5 | 0.55 | 11.4 | 4.3 | 59 | 38.9 | 54 | 2.7 | 1.2 | 0.01 | 8.7 | 4.1 |
| SEA0400 | 85 | 11.7 | 12 | 2 | 1.3 | 0.65 | 8.8 | 3.1 | 18 | 44.4 | 9 | 2.6 | 1 | 0.21 | 8 | 1.4 |
| Bumet | 132 | 6.1 | 8 | 1.8 | 0.5 | 0.44 | 11 | 3.1 | 29 | 37.9 | 15 | 1.6 | 1.3 | 0.28 | 8.5 | 3.5 |
| Amiloride | 162 | 11.1 | 31 | 1.8 | 0.6 | 0.11 | 8.7 | 2.7 | 50 | 28 | 20 | 1.7 | 0.5 | 0.34 | 8.8 | 3.5 |
| La3+ | 144 | 5.2 | 8 | 1.8 | 0.6 | 0.43 | 11.9 | 5.3 | 84 | 40.5 | 74 | 2.0 | 1.2 | 0.71 | 8.4 | 3.4 |
| C57Bl/6 WT | 460 | 11.1 | 66 | 1.5 | 0.8 | 10.8 | 4.9 | 548 | 51.1 | 620 | 2.1 | 1.5 | 8.7 | 4.0 | ||
| Cx -/- | 77 | 18.2 | 30 | 1.6 | 0.6 | 0.65 | 11.4 | 5.6 | 46 | 60.1 | 58 | 2.2 | 1.5 | 0.77 | 8.7 | 4.3 |
Abbreviations, see Table 1. Bumet: bumetanide; a-BT: a-bungarotoxin. Red color indicates statistically significant difference as compared to the control condition, p-values indicated apply to the amplitudes; ** 0.001 ≤ p < 0.01, *** p < 0.001.