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. 2020 Oct 26;18(11):528. doi: 10.3390/md18110528

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pareto Charts and response surface curves of the combined effects of temperature (40–70 °C), pressure (30–50 MPa), and ethanol as a co-solvent (0–30% v/v) on lutein recovery from (A,B) spray- and (C,D) freeze-dried biomass of Muriellopsis sp. (MCH35), respectively. The ± signs are interpreted in the Pareto graph according to the area of significance of factor or interaction in the experimental design and Response surface curves were made at 30 MPa for spray-dried and 50 MPa for freeze-dried microalga as an optimal pressure.