Figure 3.

Biological function of LOC441461 was examined in colon cancer cells. (A) LOC441461 expression levels were examined in eight colon cancer cell lines by using RT-PCR. (B) LOC441461 expression levels were examined in SW620 cells with and without small interfering RNA (siRNA) transfection. (C) The colony formation assay was performed in the SW620 cells after transfection with si-Loc441461 and a scrambled control for 2 weeks. The cells were fixed and stained with crystal violet solution. The relative colony formation ability was then quantified (lower panel). (D) The anchorage-independent growth of SW620 cells with LOC441461 transfection was examined using the soft agar formation assay. A graph illustrates the quantified values. All experiments were performed in triplicate, and these data were analyzed using Student’s t test. The difference was considered significant when p < 0.05.