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. 2020 Oct 28;12(11):3171. doi: 10.3390/cancers12113171

Figure 4.

Figure 4

LOC441461 knockdown suppressed SW620 cell proliferation by inducing cell cycle arrest and apoptosis. (A) LOC441461 expression levels were knocked down with si-LOC441461 transfection in SW620 cells. Cell proliferation compared with the scrambled control was measured using the CellTiter-Glo One Solution assay at various time points (0, 1, 3, and 5 days). (B) Distribution of cells in three phases of the cell cycle was examined using the image flow cytometry assay. (C) Graph of each quantified phase. (D) Cell-cycle-related protein levels were examined using the Western blotting assay in SW620 cells with and without LOC441461 knockdown. (E,F) After exposure to 2 µg/mL oxaliplatin, 5-FU, or irinotecan for 48 h, cell apoptosis was examined using Annexin V assay, and the percentage of apoptosis was quantified in colon cancer cells with LOC441461 knockdown and in control cells. All the experiments were performed in triplicate, and these data were analyzed using Student’s t test. The difference was considered significant when p < 0.05. ** Indicates difference that is considered significant between the indicated groups p < 0.01.