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qPCR analysis of Pc-bpi expression performed on anterior kidney, gills, mantle, and ganglia of P. canaliculata at 18, 25, and 30 °C. The changes in mRNA expression between control and treated animals were calculated with the 2-ΔΔCt method. p-value is reported only in the case of significant difference between control and treated samples. Each dot represents a single animal, bars represent mean +/- standard deviation.
