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. 2020 Oct 12;161(4):364–379. doi: 10.1111/imm.13258

FIGURE 9.

FIGURE 9

Irf4‐deficient CD8+ T cells fail to acquire terminal effector phenotypes in recipients with long‐term accepted allografts. B6.Rag1−/− recipients were adoptively transferred with either Irf4‐deficient or control CD8+ T cells, followed by BALB/c skin transplantation. Adoptively transferred CD8+ T cells in spleens were analysed by flow cytometry at day 100 post‐skin grafting. (A,B) Representative contour plots and bar graphs show % CD8+ T cells among CD45.2+ immune cells (A,B left bar graph), and absolute CD8+ T cells in spleens (B right bar graph). (C‐J) Contour plots were gated on the living transferred CD8+ T cells in spleens. Representative contour plots and bar graphs show % CD127−KLRG1+ (C,D), CD62L−CX3CR1+ (E,F), IFN‐γ+ (G,H), TCF1+ (I,J left bar graph) and EOMES+ (i,j right bar graph) cells among the adoptively transferred CD8+ T cells. Data are mean ± SD of n ≥ 3 animals per group. ns, no significance; *p < 0.05; **p < 0.01; ****p < 0.0001 (unpaired Student's t‐test)