Figure 4.
Annexin A3 (ANXA3) transcript abundance in vitro changes after exposure to certain immunostimulatory molecules. This reference dataset was contributed to GEO by our group. 59 Fresh whole blood samples from four healthy subjects were incubated for 6 hr at 37° with one of 18 different stimuli or left unstimulated. The RNAs were then stabilized with Tempus reagent and extracted before processing for microarray analysis. The stimulation conditions included: PAM3, Zymosan, Poly IC, E‐LPS, Flagellin, R837, CpG Type A, heat‐killed Legionella pneumophila (HKLP), heat‐killed Acholeplasma laidlawii (HKAL), and heat‐killed Staphylococcus aureus (HKSA) (all from Invivogen); IL‐18, TNF‐α, IFN‐α2b, IFN‐β, IFN‐γ (all from Peprotech); heat‐killed Escherichia coli (in house preparation), live influenza A virus and live respiratory syncytial virus (RSV). Levels of expression under stimulation by interferon (IFN‐α2b, IFN‐β, IFN‐γ) were found to be significantly higher than in all other conditions (t‐test, P < 0·05).