Table 1.

Characteristics of the public sepsis datasets used for validation

GSE ID	GSE49755	GSE49756	GSE49757	GSE30119	GSE64457	GSE25504	GSE13015	GSE26440
Title	A Transcriptomic Reporter Assay Employing Neutrophils to Measure Immunogenic Activity of Septic Patients Plasma ‐ Neutrophils, experiment I	A Transcriptomic Reporter Assay Employing Neutrophils to Measure Immunogenic Activity of Septic Patients Plasma ‐ Neutrophils, experiment II	A Transcriptomic Reporter Assay Employing Neutrophils to Measure Immunogenic Activity of Septic Patients Plasma ‐ Neutrophils, experiment III ‐	Genome‐wide analysis of whole blood transcriptional response to community‐acquired Staphylococcus aureus infection in vivo‐	Marked alterations of neutrophil functions during sepsis‐induced immunosuppression‐	Whole blood mRNA expression profiling of host molecular networks in neonatal sepsis	Genomic Transcriptional Profiling Identifies a Blood Biomarker Signature for the Diagnosis of Septicemic Melioidosis‐	Expression data for derivation of septic shock subgroups
References (PMID)	Khaenam et al. (24612859)	Khaenam et al. (24612859)	Khaenam et al. (24612859)	Banchereau et al (22496797)	Demaret et al. (27568821)	Dickinson et al. (26484146), Smith et al. (25120092)	Pankla et al. (19903332)	Wong et al. (19624809)
Samples	40	49	56	143	23	63	39	130
Species	Homo sapiens	Homo sapiens	Homo sapiens	Homo sapiens	Homo sapiens	Homo sapiens	Homo sapiens	Homo sapiens
Population age demographics	Adult	Adult	Adult	Paediatric	Adult	Paediatric	Adult	Paediatric
Population geographic location	Asia	Asia	Asia	North America	Europe	Europe and Africa	Asia	North America
Entry criteria	Cultured‐confirmed sepsis	Culture‐confirmed sepsis	Culture‐confirmed sepsis	Culture‐confirmed sepsis	Sepsis shock diagnosis	Suspicion of infection, later confirmed by positive cultures	Culture‐confirmed sepsis	Sepsis shock diagnosis
Assay type	Microarray	Microarray	Microarray	Microarray	Microarray	Microarray	Microarray	Microarray
Microarray manufacturer	Illumina	Illumina	Illumina	Illumina	Affymetrix	Illumina	Illumina	Affymetrix
Study type	In vitro (cultured with plasma for 6 hr)	In vitro (cultured with plasma for 6 hr)	In vitro (cultured with plasma for 6 hr)	In vivo	Ex vivo	In vivo	Ex vivo	In vivo
Cell Type	Neutrophils	Neutrophils	Neutrophils	Whole Blood	Neutrophils	Whole Blood	Whole Blood	Whole Blood
Group A	Uninfected	Uninfected	Uninfected	Control	Healthy Control	Neonatal Sepsis Control	Healthy	Normal Control
Group B	Septic	Septic	Septic	Staphylococcus aureus infection	Septic Patient	Infected	Melioidosis	Septic Shock Patient
ANXA3 Exp A	117	34·01	59·09	94·95	1157·40	185·87	18·77	4·17
ANXA3 Exp B	358	223·01	233·59	314·58	2789·04	506·61	253·04	24·91
B/A	3·1	6·55	3·96	3·29	2·41	2·72	11·92	2·19
t‐test	< 0·05	0·02	0·002	< 0·001	0·002	< 0·001	< 0·001	< 0·001
F‐test	< 0·001	< 0·001	< 0·001	< 0·001	0·48	< 0·001	< 0·001	< 0·001
Dataset GXB Link	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000072	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000073	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000074	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000136	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000127	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000104	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000130	http://sepsis.gxbsidra.org/dm3/geneBrowser/show/4000102
ANXA3 GXB Link	http://sepsis.gxbsidra.org/dm3/miniURL/view/Pk	http://sepsis.gxbsidra.org/dm3/miniURL/view/OP	http://sepsis.gxbsidra.org/dm3/miniURL/view/OU	http://sepsis.gxbsidra.org/dm3/miniURL/view/OL	http://sepsis.gxbsidra.org/dm3/miniURL/view/Pj	http://sepsis.gxbsidra.org/dm3/miniURL/view/ON	http://sepsis.gxbsidra.org/dm3/miniURL/view/OT	http://sepsis.gxbsidra.org/dm3/miniURL/view/OS