FIGURE 6.

SF improved the intrahepatic viral‐specific CD8⁺ T‐cell response in the HBV‐replicating mouse model. C57BL/6 mice were hydrodynamically injected with pSM2, pSM2 + SF or pSM2 + HV protein. Animals were killed at day 28 post‐injection. (A) Serum anti‐HBs antibodies were detected by ELISA. Frequencies of HBs‐ or HBc‐specific CD8⁺ T cells in the spleen (B) or liver (C, D) were detected by intracellular staining for IFN‐γ after 4.5 h ex vivo stimulation with HBs‐ or HBc‐derived peptides. n = 5–6 mice per group. Data are representative of three independent experiments. Bars: mean ± SD. *: p < 0.05; **: p < 0.01; ***: p < 0.001, statistical relevance was determined by one‐way ANOVA.