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. 2020 Oct 28;8(11):455. doi: 10.3390/biomedicines8110455

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS) validation of ten candidate predictive biomarkers of early-drug response. (A) Venn diagram for detection of candidate biomarkers by three statistical analyses (T₀ vs. T₁ and T₁–T₀ between two groups, and response/time terms in LMM). (B) Boxplot of abundance of PHOX2B, SH3BGRL3, and YWHAE at T₁ vs. T₀, as determined by LC-MRM/MS, in responders (blue color) and non-responders (red color). * The asterisk identifies the adjusted p-values that are significant at the 0.05 level.