Figure 4.

Depletion of PARPBP increases breast cancer cell apoptosis and DNA damage caused by epirubicin. (A, B) Colony-forming ability of the control, LV-shNC- and LV-shPARPBP-transfected MCF-7 (A) and MCF-7/EPI cells (B) in the absence or presence of EPI (2 μM) for 48 h. (C, D) The apoptotic rates of MCF-7 (C) and MCF-7/EPI cells (D) transfected with LV-shNC and LV-shPARPBP in the absence or presence of EPI (2 μM) for 48 h were visualized by flow cytometry. (E, F) Western blot analysis of γH2AX and BRCA1 expression in MCF-7 (E) and MCF-7/EPI cells (F) with or without transfection of LV-shNC or LV-shPARPBP after EPI treatment (2 μM) removal.

EPI, epirubicin; PARBP, PARP1 binding protein.