Table 3.

List of primers used for detecting E. coli, Salmonella spp., and tetracycline-resistance gene.

Target Gene	Primer Sequence (5′–3′)	Amplicon Size (bp)	Annealing Temperature (°C)	References
malB	F: GACCTCGGTTTAGTTCACAGA R: CACACGCTGACGCTGACCA	585	55	[60]
invA	F: ATCAGTACCAGTCGTCTTATCTTGAT R: TCTGTTTACCGGGCATACCAT	211	58	[61]
tetA	F: GGTTCACTCGAACGACGTCA R: CTGTCCGACAAGTTGCATGA	577	57	[62]