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. 2020 Oct 26;11(11):1259. doi: 10.3390/genes11111259

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Volcano map of differential metabolites between the pre-activated sperm and activating sperm (A), pre-activated sperm and post-activated sperm (B). Each point in the figure represents a metabolite. Green dots represent down-regulated metabolites, so the number of down-regulated metabolites in A and B is 131 and 92, respectively. Red dots represent up-regulated metabolites, so the number of up-regulated metabolites in A and B is 3 and 2, respectively. Grey dots represent identified but insignificantly different metabolites.