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A schematic representation of wild-type blocking PCR. Wild-type variant is marked by blue, mutant—by red. AS-forward, reverse primer and one WT-blocking primer constitute the PCR reaction mixture. On the 3′-terminus of the blocking primer, inhibiting three PG groups have been attached to prevent extension by DNA polymerization. The AS-primer overlapped with the blocking primer at the target mutation site and was in competition with the blocking primer.
