Table 2.
AS-PCR KRAS mutation detection using WT DNA (total 2 × 104 copies per reaction) and 1% mutant DNA in the background of WT DNA.
| Primers | Cq | ||
|---|---|---|---|
| WT | 1% | ||
| G12V | CTAT | 33.4 ± 0.1 | 31.9 ± 0.1 |
| C*TAT | 38.7 ± 0.3 | 33.2 ± 0.1 | |
| *CTAT | N/A | 40.2 ± 1.3 | |
| G12D | CGGA | 35.8 ± 0.1 | 32.50 ± 0.05 |
| C*GGA | 41.7 ± 1.8 | 32.9 ± 0.1 | |
| *CGGA | N/A | 42.9 ± 1.2 | |
| G12A | CTTC | 37.7 ± 0.3 | 32.0 ± 0.2 |
| С*TTC | N/A | 32.7 ± 0.2 | |
| G13D | GAGA | 36.3 ± 0.1 | 32.3 ± 0.1 |
| G*AGA | N/A | 35.0 ± 0.1 | |
| *GAGA | N/A | 39.2 ± 0.3 | |
No template control (NTC) was undetermined in all the reactions, N/A indicates that no Cq was obtained for a typical 45-cycle reaction. Symbol “*” means PG modification location. Boldly marked nucleotides represent mismatched nucleotides in relation to the WT DNA sequence.