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. 2020 Nov 26;10:20646. doi: 10.1038/s41598-020-77678-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Motor behavioral deficits in stroke mice. (A)–(C) Represent the similar performances of sham and HI mice in the open field test (OFT) at 3 weeks after stroke. Parameters of the OFT include the total distance (A), the mean velocity (B) and the time spent in the center zone (C). N = 8 mice per group in (A)–(C). (D) Quantitative measurements showed comparable performances of sham and HI mice in the rotarod test. N = 14 mice in sham and N = 10 in stroke group. (E,F) Show the motor deficits of HI mice in the taper beam and wire hanging tests. (E) Increased fault numbers of stroke mice in tapered beam test at 24 h and 3 weeks after stroke than sham group. N = 19 mice per group. (F) Decrease in holding time of HI mice in the wire hanging test at 48 h post ischemia and remaining to 3 weeks after stroke. N = 19 mice per group. Data are shown as the mean and SEM for each group. * Compared with sham group, P < 0.05, **P < 0.01, ***P < 0.001, unpaired t-test in (A)–(C); two-way repeated ANOVA followed by fisher multiple comparison test in (D)–(F).