. 2020 Nov 4;12(11):3245. doi: 10.3390/cancers12113245

Table 2.

Immune subsets and their corresponding phenotypes analyzed in bone marrow (BM) and peripheral blood (PB) samples.

Immune Subset	Expression of Markers
NGF MRD panel
Plasma cells	CD38^brCD138+
B cells	CD19+CD45+
Naïve B cells	CD19+CD27-CD38^-/dimCD45+SSC^low
B cell precursors	CD19+CD27-CD38^brCD45^dimSSC^low
Memory B cells	CD19+CD27+CD38^-/dimCD45+SSC^low
T cells	CD19-CD45+CD56-SSC^low
CD27+ T cells	CD19-CD45+CD56-CD27+SSC^low
NK/NKT cells	CD19-CD45+ CD56-SSC^low
CD27+ NK/NKT cells	CD19-CD45+CD56-CD27+SSC^low
Neutrophils	CD45^dimSSC^high
Myeloid progenitors	CD38+CD45^dimCD117+SSC^high
Monocytes—TAMs	CD38+CD45+CD81+SSC^int
Mast cells	CD45^dimCD117^br
Erythroblasts	CD38-CD45-SSC^low
Erythroid progenitors	CD38^-/dimCD45^-/dimCD117+SSC^low
T cell panel
T regulatory cells (Tregs)	CD3+CD4+CD25+CD127^lowFoxP3+
Effector/effector memory Tregs (eff/eff mem Tregs)	CD3+CD4+CD25+CD127^lowFoxP3+CD45RA-CD45RO+HLA-DR-CTLA4+
Terminal effector Tregs (teff Tregs)	CD3+CD4+CD25+CD127^lowFoxP3+CD45RA-CD45RO+HLA-DR+ CTLA4+
Resting Tregs	CD3+CD4+CD25+CD127^lowFoxP3+CD45RA-CD45RO+HLA-DR-CTLA4-
CD39+ suppressor Tregs (CD39 Tregs)	CD3+CD4+CD25+CD127^lowFoxP3+CD45RA-CD45RO+CD39+
CD4+ Τ cells	CD3+CD4+
Naïve CD4+ Τ cells	CD3+CD4+CD45RA+CD45RO-
Effector/Effector memory CD4+T cells (eff/eff mem CD4+)	CD3+CD4+CD45RA-CD45RO+
CD8+ T cells	CD3+CD8+
CD8+ Tregs	CD3+CD8+CD25+FoxP3+
Memory CD8+ T cells	CD3+CD8+CD45RO+
HLA-DR regulatory CD8+ T cells (HLA-DR reg CD8+)	CD3+CD8+HLA-DR+
MDSC panel
Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs)	CD14-CD11b+CD15+SSC^high
Early myeloid-derived suppressor cells (eMDSCs)	Lin(CD3/CD14/CD15/CD19/CD56)-HLA-DR-CD33+
Monocytic myeloid-derived suppressor cells (M-MDSCs)	CD11b-CD14+HLA-DR^low/-CD15-
M1 monocytes	Lin(CD3/CD14/CD15/CD19/CD56)-CD14+CD124-
M2 monocytes	Lin(CD3/CD14/CD15/CD19/CD56)-CD14+CD124+

Abbreviations: br: bright expression; int: intermediate expression; SSC: side scatter.