Figure 3.

(a) Effect of mouse monoclonal antibody against DON (anti-DON mAb) concentration on the zero calibrator signal received from chips coated with 100 (white columns), 200 (light grey columns), or 400 μg/mL DON conjugate with ovalbumin (DON-OVA) (dark grey columns). (b) Effect of anti-DON mAb concentration on the percent signal corresponding to calibrators containing 10 ng/mL (light grey columns) and 100 ng/mL DON (dark grey columns) with respect to zero calibrator signal (white columns) obtained from a chip coated with 200 μg/mL of DON-OVA conjugate. In all cases, the duration of both primary and secondary immunoreaction was 10 min. Each point is the mean value of 3 measurements ± SD.