Figure 4.

(a) Effect of primary immunoreaction duration on the zero calibrator signal without (open squares) or with an additional 5-min reaction with an anti-mouse IgG antibody (closed squares). Each point is the mean value of 3 measurements ± SD. (b) Real-time response corresponding to zero DON calibrator when running over a chip coated with a 200 μg/mL DON-OVA solution: assay buffer (start to arrow 1; 2 min), 1:1 (v/v) calibrator/anti-DON mAb mixture (arrow 1 to 2; 7 min), and anti-mouse IgG antibody (arrow 2 to 3; 30 min). The dashed red lines indicate the signal received for running the anti-mouse IgG antibody for 5 min.