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. 2020 Nov 26;10:20703. doi: 10.1038/s41598-020-77656-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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cDPSCs contained a superior osteogenic differentiation potential upon cBM-MSCs in vitro. Schematic diagram of an in vitro osteogenic induction and the analyses of the osteogenic differentiation potential was showed (A). ALP activity at day 14 (B), matrix mineralization by Von Kossa staining with mineralized area percentage at day 7 and 14 (C), and osteogenic mRNA marker expression at day 7 and 14 (D) of cBM-MSCs and cDPSCs were investigated (n = 4). ALP activity was normalized with undifferentiated control. Relative mRNA expression was normalized with the reference gene, Gapdh, and undifferentiated control. Bars and asterisks indicated the significant difference comparing with control group (p value < 0.05).