Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 26;10:20703. doi: 10.1038/s41598-020-77656-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Trend of signaling protein expression and functional validation of selected potential signaling regulating osteogenic differentiation by cBM-MSCs and cDPSCs in vitro. Pie charts represented trend of signaling protein expression by cBM-MSCs and cDPSCs upon an in vitro osteogenic differentiation at day 7 and 14 post-induction (A). Signaling categories and numbers of protein were mentioned. Schematic diagram of an in vitro osteogenic induction and functional validation of selected potential signaling on osteogenic differentiation was showed (B). Functional validation of the potential signaling regulating an in vitro osteogenic differentiation by cBM-MSCs (C) and cDPSCs (D) was performed by treatment with specific signaling inhibitors (canonical Wnt inhibitor: Dkk-1, Notch inhibitor: DAPT, TGF-beta inhibitor: SB431542, and BMP inhibitors: noggin and dorsomorphin). Assessment of matrix mineralization by Von Kossa staining with mineralized area percentage was performed at day 7 and 14 post-induction (n = 4). Asterisks and sharps indicated the significant difference comparing with undifferentiated control and osteogenic control, respectively (p value < 0.05).