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. 2020 Nov 26;10:20657. doi: 10.1038/s41598-020-77687-7

Figure 6.

Figure 6

F. hepatica KT inhibitors inhibit all secreted cysteine protease activity, but not somatic extract activity. (A) Cysteine protease activity within somatic extracts of adult F. hepatica were measured using the fluorogenic peptide substrate Z-Phe-Arg-NHMec (relative fluorescent units, RFU/min) in the presence of FhKT1.1 (red line), FhKT1.3 (blue line) and the cysteine protease inhibitor E-64 (black line) at a range of concentrations [I] (1 μM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.25 nM and 15.625 nM). The F. hepatica KT inhibitors do not completely inhibit all cysteine protease within the somatic extract. (B) Cysteine protease activity within the ES proteins of adult F. hepatica measured in the presence of FhKT1.1 (red line) and FhKT1.3 (blue line) at a range of concentrations [I] (1 μM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.25 nM and 15.625 nM). Graphical representations produced using GraphPad Software (v6 for Windows).