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. 2020 Nov 26;10:20657. doi: 10.1038/s41598-020-77687-7

Table 1.

Inhibition constants (K_i) for the FhKT recombinant protein against cysteine and serine proteases inhibited.

Enzyme	Inhibition constant (K_i), in nM
Enzyme	rFhKT1^a	rFhKT1Leu¹⁵/Arg¹⁵	rFhKT1.3	rFhKT1Arg¹⁹/Ala¹⁹
Cysteine proteases
F. hepatica Cathepsin L1	0.4 (± 0.1)^a	0.7 (± 0.04)^a	0.6 (± 0.2)	4.2 (± 0.4)
F. hepatica Cathepsin L2	10 (± 0.3)^a	27 (± 1.5)^a	10.6 (± 0.3)	35 (± 3.9)
F. hepatica Cathepsin L3	1.8 (± 0.6)	3.6 (± 0.3)	3.2 (± 0.2)	32.8 (± 7)
Human Cathepsin L	1.6 (± 0.1)^a	3 (± 0.1)^a	2.6 (± 0.5)	0.3 (± 0.004)
Human Cathepsin K	5 (± 0.3)^a	5 (± 0.3)^a	5.5 (± 0.3)	18.5 (± 2.9)
Serine protease
Bovine Trypsin	N.I	1.5 (± 0.7)^a	1.8 (± 0.2)	N.I

N.I not inhibited.

^aAs reported by Smith et al.¹⁰.