Table 2.

The selection of 23 non-redundant dsDNA sequences (as templates for putative aptamers against the RBSDV His-P10 protein) from 50 T-A cloning constructs at SELEX round 10.

Serial No.	T-A Clone No.	Sequence (5′-3′)	nt
1	1	TGACACCGTACCTGCTCTAGATGAAGACTGATGATGCCTGTCAACGCGCGGAACCACTGGATCAAGACGGACCGGAACCGTGTTCACTGTCGACAAGCACGCCAGGGACTAT	118
2	3	TGACACCGTACCTGCTCTAGCGGTCTCGTGAATCTGCAGACGAATTTGTGTAATATGAGCGCATATGTATAACATTGCAGACAAGGTCGAGAGCGCGTAAGCACGCCAGGGACTAT	122
3	4	TGACACCGTACCTGCTCTGGGTGTGGAGATTGTGAGGGGAGGTGCTAGCATGGATAGAAAGTAAAGAGGAGGAAAGTGCGGAGTAAGGAGGCGGGGAAGCACGCCAGGGACTAT	120
4	5	TGACACCGTACCTGCTCTGACAGTCCTCGTACAAAGGTCGAGTCATCATACCCGCCGTAACCCCTTACGGGTCGAGCCAAGCACGCCAGGGACTAT	102
5	6	TGACACCGTACCTGCTCTCAGGGCAACACTATGACATAGGGAACTCTCGGAACACAGGGAGGCCTCCAGTACAATTCGGATGTGAACTGCAAGCACGCCAGGGACTAT	114
6	11	TGACACCGTACCTGCTCTAGCACGGCACCCATGGGCAGTATACCGTCCCCCGCGAAAGACGGGCCGCTGCGGTAAGCACGCCAGGGACTAT	97
7	13	TGACACCGTACCTGCTCTACCCCGCATTATCCTTATAACCCGATAGAATAAACGACGAGGTCGGCGAAAGACCGAAGGCGGTGCAGGGCCAAGCATGCAAGCACGCCAGGGACTAT	122
8	15	TGACACCGTACCTGCTCTAGCCACCTTCGTAGACACCATATCAGAAAGAGATACCCAGGAGGCGCCCCCGTGTGCGAACGAAGGGCGATTGTAACGAAGCACGCCAGGGACTAT	120
9	16	TGACACCGTACCTGCTCTGGGCCGTTGATTCCAACCCTTTATGGCGGCGTGCGAAAGACACGATCAACGCGCAGCCCCAAGCACGCCAGGGACTAT	102
10	17	TGACACCGTACCTGCTCTGCCGTAGTGATCCTCCTTAACAGTGACCTATAGATCGTTCGTCGTATTTGCTTTGACAATCCTTCTCAGATACTCTCCCGAAGCACGCCAGGGACTAT	122
11	20	TGACACCGTACCTGCTCTAGTCGTGGTCATGCAGCACGGAAGCGTAAGACTTCCCGCTGGACGGTCGGTAAGACGGTAAATGACTCCGCAAGCACGCCAGGGACTAT	113
12	21	TGACACCGTACCTGCTCTCTACCAGCTCCGACGATCCAGTATTGTGAACCCCGCGCGGTAGCGTTCTCGTCTTCGTGGAGTGGAACGCAAGCACGCCAGGGACTAT	112
13	22	TGACACCGTACCTGCTCTGCCCGTAGACAAAGTCCGCCCCGAAATCGCAAGACTGCAAGCGAAAGACTAAAGCGATCGAATAAGATCCGATTCAGGGGAAAGCACGCCAGGGACTAT	123
14	24	TGACACCGTACCTGCTCTGCCGTAGTGATCCTCCTTAACAGTGACCTATAGATCGTTCGCCGTATTTGCTTTGACAATCCTTCTCAGATACTCTCCCGAAGCACGCCAGGGACTAT	122
15	25	TGACACCGTACCTGCTCTGGGCCGTTGATTCCAACCCATTATCGCGGCGTGCGAAAGACACGATCAACGCGCAGCCCCAAGCACGCCAGGGACTAT	102
16	26	TGACACCGTACCTGCTCTGGGTCAGCGAAAGACTACCCCCGTGGCGCGCGGAAGACGCGCCCAGCAGGACCAGCCGAACACCCCAAGCACGCCAGGGACTAT	108
17	27	TGACACCGTACCTGCTCTAGGGAGTCGATGTTCCCTGCTCACGACATGGTCCGCGGAAGACGGACAGGACATCCCGAGAAGCGAGCGAGCATCTCGCTAAGCACGCCAGGGACTAT	122
18	29	TGACACCGTACCTGCTCTACCTGGATGCGCGTTAGACGCTGTTGAATAATCATCGCAAAGAGTCCAGGTACGCAAGGTTATGGACAGTGTGCCAAGCACGCCAGGGACTAT	117
19	32	TGACACCGTACCTGCTCTAGAACGCAGCTCAAAAGCTCGCCCAGCACCCGAAAAGAGGTGCGGCCGTCTGAGGGAAGCAGCGCAGAACCCCGAAGCACGCCAGGGACTAT	116
20	34	TGACACCGTACCTGCTCTGGAGACGAAGAGATAAGGCAGAAGTTGAGAAGATGGGATGAGGGCCGGGAAGCACGCCAGGGACTAT	91
21	36	TGACACCGTACCTGCTCTACCCCGCATTATCCTTATAACCCGATAGAATAAATGACGAGGTCGGCGAAAGACCGAAGGCGGTGCAGGGCCAAGCATGCAAGCACGCCAGGGACTAT	122
22	38	TGACACCGTACCTGCTCTGGGCCGTTGATTCCAACCCTTTATCGCGGCGTGCGAAAGACACGATCAACGCGCAGCCCCAAGCACGCCAGGGACTAT	102
23	45	TGACACCGTACCTGCTCTGGGCGGTGCGTCGCTTTGCTGAAGCCAGATTGGTCTTTGACAGAGATCAGACAGAACTGTTCCAGAAAGCGAGGGAAGCACGCCAGGGACTAT	117

The primer set flanking the N80 ssDNA library was adopted from Schütze’s previous paper [38] to be devoid of hairpin, dimer, and heterodimer-forming sequences. The forward primer region (blue) and reverse primer region (red) of the screened-out aptamers are highlighted in Table 2, respectively.