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. 2020 Oct 29;9(11):2370. doi: 10.3390/cells9112370

Figure 2.

Figure 2

Biophysical analysis of suramin binding to NF-Yd; (a) Suramin binding to purified NF-Yd proteins measured by isothermal titration calorimetry (ITC). Upper panel, heat changes (µcal/s) during successive injections of 8 µL of suramin (250 µM) into the chamber containing NF-Yd (20 µM). Lower panel, binding curve obtained from data displayed in the upper panel. The peaks were integrated and normalized per mole of suramin injected. Both panels are plotted against the molar ratio suramin:NF-Yd. The solid line represents a nonlinear least-squares fit to a double dependent sites binding model (see Materials and Methods). The experiment was repeated three times; (b) gel-filtration analysis (Superdex 75 10/300 GL column, GE Healthcare, Uppsala, Sweden) of the oligomerization state of NF-Yd induced by different molar ratios of suramin.