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The detection of PT modification. (A) ICA analysis of gDNA. Lanes 1–2, genomic DNA from E. coli B7A with (+) or without (-) iodine. Lanes 3–4, genomic DNA from E. coli DH10B with (+) or without (-) iodine. (B) and (C) LC-MS/MS analysis of the PT-linked dinucleotides from E. coli B7A and from E. coli DH10B. The PT-modified dinucleotide (GpsA or GpsT) was used as standards.
