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. 2020 Oct 24;9(11):879. doi: 10.3390/pathogens9110879

Figure 3.

Figure 3

Ischemic pre (IPC)- and post-conditioning (IPO) induce upregulation of canonical and non-canonical inflammasome regulators more than liver IRI. A database mining work (GSE24430) of rat liver ischemia-reperfusion with the effects of ischemic pre- (IPC) and post-conditioning (IPO). (A) Schematic presentation of experimental design for the microarray analysis [55]. IPC (10 min ischemia/10 min reperfusion before ischemia for 30 min/reperfusion for 30 min) and IPO [(0.5 min ischemia/0.5 min reperfusion) × 3) after 30 min ischemia]. (B-1) The detailed description of group classification and a summary of pyroptosis gene changes (up-/downregulation) in each group. (B-2) The Venn-diagram of up and downregulated genes shown in (B-1). (B-3) The details of overlapped gene groups, gene names, and fold changes (Log2FC) of each gene. To Note: These up and downregulated genes were significantly changed compared to the sham group (p value < 0.05). Non-canonical genes were marked in green. (C) We used the GENEONTOLOGY website to analyze the four groups (IRI, IPC, IPO, IPC + IPO) of genes in B-1 and found the top five-fold enrichment pathways that are related to each of the group entities. The gene list of each group were listed in Supplementary Table S5. (D) The Venn diagram of the four group pathways. (E) The overlapped pathway among the four groups. Only one pathway—the type I interferon biosynthetic process—was held by both the IPC and IPC+IPO groups. The other pathways were exclusive in each group.