Figure 1.

The TRPM6 channel mediates the Mg influx in human colon carcinoma cells. CaCo-2 cells were transiently silenced for TRPM6 (siRNA) and assessed 48h after transfection. Control cells (CTRL) received non-silencing scrambled siRNA. (a) Mg influx capacity in TRPM6-silenced (open circles) vs. control cells (solid circles), as assessed by the live imaging of Mag-Fluo-4-loaded cells; a representative experiment is shown. (b) TRPM6 protein expression in TRPM6-silenced and control cells, as evaluated by Western blot analysis; a representative blot is shown. (c) Quantification of TRPM6 protein expression by Western blot densitometry normalized to β-actin levels (n = 3, mean ± SD) in TRPM6-silenced (white bar) and control (black bar) cells. ** p < 0.01 by paired Student’s t-test. Full scans of original blots are available in Figure S1.