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. 2020 Oct 24;9(11):355. doi: 10.3390/biology9110355

Figure 2.

Figure 2

Multiple alignment among the sequences analyzed in Figure 1 as obtained by Clustal Omega (default parameters). EF-hand, calcium-binding motifs (Efh) are highlighted in grey. Asterisks (*) correspond to single, fully conserved residues. Colons (:) indicate conservation of residues sharing strongly similar properties (equivalent to scoring > 0.5 in the Gonnet PAM 250 matrix). Periods (.) indicate conservation of residues with weakly similar properties (equivalent to scoring ≤ 0.5 and > 0 in the Gonnet PAM 250 matrix).