Table 6.

Target genes and primers used in the study.

Pathogen	Target Gene	Primer Sequence (5′->3′)	Annealing Temp (°C)	Amplicon Size (bp)	Reference
Babesia ovis	ssu rRNA	TGGGCAGGACCTTGGTTCTTCT CCGCGTAGCGCCGGCTAAATA	62	549	[61]
Theileria spp.	18S rRNA	GAAACGGCTACCACATCT AGTTTCCCCGTGTTGAGT TTAAACCTCTTCCAGAGT TCAGCCTTGCGACCATAC	55	778 581	[62]
Theileria ovis	ssu rRNA	TCGAGACCTTCGGGT TCCGGACATTGTAAAACAAA	53	520	[52]
Theileria lestoquardi	18S rRNA	GTGCCGCAAGTGAGTCA GGACTGATGAGAAGACGATGAG	52	730	[43]
Anaplasma ovis	msp4	TGAAGGGAGCGGGGTCATGGG GAGTAATTGCAGCCAGGCACTCT	62	347	[63]
Anaplasma phagocytophilum	16S rRNA	TCCTGGCTCAGAACGAACGCTGGCGGC AGTCACTGACCCAACCTTAAATGGCTG GTCGAACGGATTATTCTTTATAGCTTGC CCCTTCCGTTAAGAAGGATCTAATCTCC	50	1433 926	[64]
Ehrlichia ruminantium	pCS20	ACTAGTAGAAATTGCACAATCYAT RCTDGCWGCTTTYTGTTCAGCTAK ACTAGTAGAAATTGCACAATCYAT TGATAACTTGGWGCRRGDARTCCTT	61	400 278	[65]