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. 2020 Oct 28;10(11):785. doi: 10.3390/brainsci10110785

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Experimental timeline of adolescent intermittent ethanol (AIE) and behavior tests across the lifespan. Long Evans rats underwent intermittent gavage with either water (CON) or 5 g/kg ethanol (AIE) across adolescence (postnatal day (PND) 25–54). After two months of rest, rats began a battery of behavioral tests that included spatial (radial arm water maze, Hebb-Williams maze) and non-spatial (novel object recognition) memory tasks as well as an assessment of dominance behaviors (tube dominance test). In every test, females and males were tested separately. Rats were allowed at least two weeks of recuperation between each test. There were 14 CON rats (n = 8 male, n = 6 female) and 13 AIE rats (n = 7 male, n = 6 female).