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. 2020 Oct 31;12(11):3216. doi: 10.3390/cancers12113216

Figure 5.

Figure 5

Targeting NHEJ-associated enzymes seems generally potent to sensitize 3D PDAC cell cultures to photon and proton irradiation. (A) 3D tumoroid formation capacity of unirradiated indicated PDAC cells treated with indicated DNA repair inhibitors (experimental set-up shown in Figure S2A); (B) Normalized 3D PDAC tumoroid formation capacity upon 1-h inhibitor pretreatment combined with 6-Gy photon or proton irradiation; (C) Differences in in the radiosensitizing efficacy of inhibitors visualized by Δ values of normalized tumor formation capacity (tumoroid formation capacity after 6 Gy of protons - tumoroid formation capacity after 6 Gy of photons); (D) Enhancement ratio (tumoroid formation capacity after 6 Gy control treatment/tumoroid formation capacity after 6 Gy inhibitor treatment) of each cell line analyzed in B (see Figure S6) after photon and proton irradiation plotted logarithmically; (E) Analysis of differences in the radiosensitizing efficacy of indicated inhibitors showing violin blots of summarized Δ values of tumoroid formation capacity from all PDAC cell lines analyzed in Figure S6. All results show mean ± SD (n = 3; two-sided t-test; *, p < 0.05; **, p < 0.01; ***, p < 0.001); Δ: delta, TF: tumoroid formation.