Figure 3.

Electron microscopy investigations were carried out to evaluate morphological changes induced by LEO and NanoLEO treatments (a) Scanning electron microscopy (SEM) micrograph of untreated Caco-2 cells at low magnification; (b) SEM micrograph of cellular protrusions of untreated Caco-2 cells membranes; (c) SEM micrograph of Caco-2 cells treated for 4 h with LEO; (d) A particular of the LEO treated Caco-2 cells surface by SEM; (e) SEM micrograph of NanoLEO treated Caco-2 cells where nanoemulsions particles were observed (arrow heads); (f) Nanoemulsion globules with a round shape; (g) transmission electron microscopy (TEM) micrograph of nanoemulsion treated cells. Arrow heads evidence a particle; (h) Negative staining technique shows NanoLEO globules with a different diameter. Bars = (a,c,e) 5 µm; (b,d,f,h) 1 µm; (g) 2µm.