Table 2.
LEO and NanoLEO EC50 values obtained by dose-dependent MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay after 24, 48 and 72 h of treatments. The values are expressed as mean ± SD. Vinblastine was used as positive control 1. p < 0.05 according to ANOVA test.
| Cell Line | Time | EC50 % of EO (Mean ± SD) | |
|---|---|---|---|
| LEO | NanoLEO | ||
| CCRF-CEM | 24 h | 1.30 × 10−2 ± 0.09 × 10−2 | 0.57 × 10−2 ± 0.08 × 10−2 |
| 48 h | 1.80 × 10−2 ± 0.37 × 10−2 | 0.47 × 10−2 ± 0.08 × 10−2 | |
| 72 h | 1.25 × 10−2 ± 0.08 × 10−2 | 0.87 × 10−2 ± 0.05 × 10−2 | |
| SHSY5Y | 24 h | 1.42 × 10−2 ± 0.08 × 10−2 | 0.64 × 10−2 ± 0.05 × 10−2 |
| 48 h | 1.35 × 10−2 ± 0.08 × 10−2 | 0.62 × 10−2 ± 0.05 × 10−2 | |
| 72 h | 1.97 × 10−2 ± 0.10 × 10−2 | 0.44 × 10−2 ± 0.02 × 10−2 | |
| Caco2 | 24 h | 13.52 × 10−2 ± 1.97 × 10−2 | 1.79 × 10−2 ± 0.53 × 10−2 |
| 48 h | 24.12 × 10−2 ± 0.60 × 10−2 | 1.33 × 10−2 ± 0.28 × 10−2 | |
| 72 h | 12.45 × 10−2 ± 0.99 × 10−2 | 1.58 × 10−2 ± 0.09 × 10−2 | |
| MCF-7 | 24 h | 3.62 × 10−2 ± 0.76 × 10−2 | 1.73 × 10−2 ± 0.10 × 10−2 |
| 48 h | 10.25 × 10−2 ± 2.33 × 10−2 | 1.47 × 10−2 ± 0.20 × 10−2 | |
| 72 h | 4.53 × 10−2 ± 0.68 × 10−2 | 1.35 × 10−2 ± 0.21 × 10−2 | |
| MCF-10A | 24 h | 6.73 × 10−2 ± 1.04 × 10−2 | 0.90 × 10−2 ± 0.14 × 10−2 |
| 48 h | 4.28 × 10−2 ± 0.51 × 10−2 | 0.60 × 10−2 ± 0.02 × 10−2 | |
| 72 h | 3.47 × 10−2 ± 0.06 × 10−2 | 0.25 × 10−2 ± 0.14 × 10−2 | |
1 EC50 values of Vinblastine: CCRF-CEM 24.11 ± 4.02 nM; SHSY5Y 1.92 to ± 0.14 nM; Caco2 3.74 × 103 ± 0.13 × 103; MCF-7 2.62 × 103 ± 0.38 × 103 nM; MCF-10 ≥ 250 × 103 nM.