Figure 1.

Morphological analysis of cytoskeleton and nuclei in MG63 cells grown for 48 h in the presence of DM nanoparticles and DM/n-HA (2:1) nanocomposites. Fluorescence imaging was performed by using Phalloidin-FITC (green fluorescence) and DAPI (blue) for staining cytoskeleton and nuclei, respectively. The morphology of cytoskeletal structures and nuclear shape has been detected in untreated control cells (A), DM-treated cells (B) and DM/n-HA-treated cells (C). Image capture was performed by a Zeiss LSM 710 confocal microscopy system, equipped with the ZEN 2009 software (LSM 710 suite) [scale bar: 50 µm; magnif. 40X].