Skip to main content
. 2020 Oct 25;9(11):357. doi: 10.3390/biology9110357

Table 1.

Details of primer sequences for the qPCR assays. For each gene, the NCBI accession number of the mRNA reference sequence (Ref Seq mRNA; rDNA for the 28S ribosomal gene) used for primer design is reported. Gene-specific nucleotide sequences are reported as forward (sense) and reverse (antisense) primers on the 5′-3′ direction. For each primer pair, the expected amplicon length is reported (PCR product size) as base pairs (bp). Human genes are listed with the official symbols/aliases.

Human Gene Ref Seq mRNA
acc. n.
5′-3′ Sense 5′-3′ Antisense PCR Product Size (bp)
SLC11A2/DMT1 NM_001174125.1 CACTGTGAACTAAAATCAT CTCCTCCTCAGGAATGGAGA 235
P53 NM_000546.5 CCTCCTCAGCATCTTATCCG GCACAAACACGCACCTCAAA 250
FAK NM_005607.4 ATTAAATGGATGGCTCCAGA CTCCCACATACACACACCAA 89
28S M27830.1 GACGAGAGGGCGTGCATTC TAAAATCCCGCGGACGCAAA 138