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. 2020 Aug 19;228(5):1627–1639. doi: 10.1111/nph.16826

Fig. 4.

Fig. 4

TDP1 is required for AN‐mediated transcriptional repression on MYB46 in Arabidopsis. (a) ChIP‐qPCR analysis on an‐t1 and tdp1‐2 backgrounds. Upper scheme indicates primers for the amplification of MYB46 promoters (MYB46 P1 and P2). Nontransgenic plants (Col‐0) and no antibody assays were used as negative controls. (b) Transactivation analysis showing that TDP1 enhances AN transcriptional repression on MYB46. Left scheme displays the four vectors used in transactivation analyses: reporter construct containing MYB46 promoter and GUS reporter gene; transactivation construct expressing VND6; effector construct expressing AN; effector construct expressing TDP1. GUS activity in individual assays was normalised against luciferase activity (GUS/LUC). (c) µChIP‐qPCR analysis demonstrating the enhancement of association of AN with MYB46 promoter by TDP1. Assays without antibody (no ab) were performed as negative controls. Values represent means ± SE, n = 3. Statistical significance was determined by two‐tailed Student’s t‐tests (**, P < 0.01; *, P < 0.05; ns, P > 0.05).