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. 2020 Nov 26;8(2):e001128. doi: 10.1136/jitc-2020-001128

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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PODO447 recognizes a glycomotif on Podxl’s mucin domain. (A) Flow cytometric histograms of SKOV3 wild-type (WT) cells treated with either assay buffer (control), neuraminidase (which removes the terminal sialic residues) or O-sialoglycoprotein endopeptidase (which cleaves the mucin domain). Cells were then stained with either PODO83 (blue) or PODO447 (red). (B) Flow cytometric histograms showing PODO83 (blue lines) and PODO447 (red lines) immunoreactivity to SKOV3 PODXL-KO cells re-expressing the WT podocalyxin (Podxl) (WT Podxl) protein or a Podxl-mutant lacking the mucin domain (ΔMucin Podxl).