Figure 6.

CCL22 ligand level in tumors is upregulated after CPI treatment in tumors. (A) CCL22 ligand concentration was measured in CT26 tumor lysates after treatment with checkpoint inhibitors or immune agonists days post dose (n=5). (B) Concentration of proinflammatory cytokines in the tumor was measured 3 days post randomization (n=5). (C) T_reg migration study in CT26 tumors. Mice were dosed twice with anti-CTLA-4 antibody (on the day of randomization and 3 days later). GFP⁺ T_reg were transferred 7 days post first antibody challenge. CCR4 antagonist treatment started 3 hours prior to T_reg transfer. Number of migrated GFP⁺ T_reg in the tumor (left graph) and spleen (right graph) 6 days post cell transfer (n=8). This is representative data from two independent studies. For statistical analysis, the ordinary one-way analysis of variance with Dunnet’s correction was used. CPI, checkpoint inhibitor; IFN-γ, interferon gamma; IL-1β, interleukin 1 beta; TNF-α, tumor necrosis factor alpha.