Fig. 3.

RpoS‐deficient V. cholerae are producing HapR and are naturally transformable.

(A and C) WT and rpoS‐or hapR‐minus strains were tested for natural transformation under chitin‐dependent (A) or chitin‐independent (C) conditions. Details for (A) as in Fig. 2A.

B. Strains for HapR detection by Western blotting were grown in plain LB medium and processed as those samples in Fig. 2C. For panel (C), the strains carried inducible TfoX on a transposon and were grown in LB medium in the absence (0%), or presence (0.02% or 0.2%) of arabinose as an inducer as indicated above the graph. All experiments were done three independent times and their average is shown in the bar graphs (±SD). <, below detection limit. Statistical analyses: (A) one‐way ANOVA with Tukey's multiple comparisons test. The detection limit was used for statistical analysis for conditions in which no transformants were recovered. ****P < 0.0001; ns, not significant.