Fig 3.

An insertional deletion of Hel increases the proportion of ICE‐free form. Relative quantities detected by qPCR of Chr, Circ, and Scar forms. First replicate of wild type cultures has been normalized to 1 on each form. Asterisks indicate significant statistical differences (P‐value < 0.05) between wild type and mutant ratios under the same conditions for the detection of the same ICE form. Samples have been normalized relative to RNA polymerase alpha subunit, DNA Polymerase III and 16S genes (n = 3).