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Absence of both Ppol and ThAgo sharply increases ICETh2 excision. Relative quantities detected by qPCR of Chr, Circ, and Scar forms. First replicate of wild‐type cultures has been normalized to 1 on each form. Asterisks indicate significant statistical differences (*P‐value < 0.05 and **P‐value < 0.01) between wild type and mutant ratios for the detection of the same ICE form. Samples have been normalized relative to RNA polymerase alpha subunit, DNA Polymerase III and 16S genes (n = 3).
