Figure 7.

(a) RAW264.7 cells were left untreated or treated for 24 h with SB3-14 at the indicated concentrations. Cells were stained using the PerCP–Annexin V and FVD 780 and analyzed by flow cytometry. Annexin V/FVD—double negative cells (lower left quadrant) represented live cells, annexin V/FVD—double positive cells (upper left and upper right quadrant) represented apoptotic cells, and annexin V-negative/FVD-positive cells (lower right quadrant) indicated dead cells. A representative dot plot (relative to untreated cells) is shown. The percentage of viable, apoptotic, and dead cells was reported in (b) for each SB3-14 concentration. Data are the mean percentage of two different experiments.